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90
OriGene anti adenosine a3 receptor abs
FIGURE 4. The enhanced inhibitory effect of NECA is mediated by the enhanced contribution of A2AR-mediated signaling and the decreased contribution of A3R-mediated signaling. Unstimulated and activated microglia were exposed to 100 ng LPS/ml in the presence or absence of 10 M NECA in combination with A1, A2A, A2B, and <t>A3</t> receptor antagonists. After 16 h, TNF- (A) and IL-12p40/p70 (B) levels were measured and expressed as relative values of exposure to 100 ng LPS/ml alone. DPCPX was used as an A1R, SCH58261 as an A2AR, alloxazine as an A2BR and VUF5574 as an A3R antagonist. All antagonists were used at 10 M concentrations. Solid lines in the graph indicate relative cytokine production levels of exposure to 100 ng LPS/ml 10 M NECA without antagonists of unstimulated microglia, the dashed lines indicate activated microglia. , p 0.05; Student’s t test. TNF- (C) and IL-12p40/p70 (D) production after exposure to 100 ng LPS/ml in the presence or absence of 10 M CGS21680, an A2AR-selective agonist, and/or of 10 M VUF5574, an A3R antagonist. Cytokine production is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. , p 0.05; Student’s t test. TNF- (E) and IL-12p40/p70 (F) production after exposure to 100 ng LPS/ml different concentrations of CGS21680, in the presence or absence of A3R antagonist, is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. All data are representative for three independent experiments using different donors.
Anti Adenosine A3 Receptor Abs, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Data Translation Inc image process and analysis software sp0550
FIGURE 4. The enhanced inhibitory effect of NECA is mediated by the enhanced contribution of A2AR-mediated signaling and the decreased contribution of A3R-mediated signaling. Unstimulated and activated microglia were exposed to 100 ng LPS/ml in the presence or absence of 10 M NECA in combination with A1, A2A, A2B, and <t>A3</t> receptor antagonists. After 16 h, TNF- (A) and IL-12p40/p70 (B) levels were measured and expressed as relative values of exposure to 100 ng LPS/ml alone. DPCPX was used as an A1R, SCH58261 as an A2AR, alloxazine as an A2BR and VUF5574 as an A3R antagonist. All antagonists were used at 10 M concentrations. Solid lines in the graph indicate relative cytokine production levels of exposure to 100 ng LPS/ml 10 M NECA without antagonists of unstimulated microglia, the dashed lines indicate activated microglia. , p 0.05; Student’s t test. TNF- (C) and IL-12p40/p70 (D) production after exposure to 100 ng LPS/ml in the presence or absence of 10 M CGS21680, an A2AR-selective agonist, and/or of 10 M VUF5574, an A3R antagonist. Cytokine production is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. , p 0.05; Student’s t test. TNF- (E) and IL-12p40/p70 (F) production after exposure to 100 ng LPS/ml different concentrations of CGS21680, in the presence or absence of A3R antagonist, is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. All data are representative for three independent experiments using different donors.
Image Process And Analysis Software Sp0550, supplied by Data Translation Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+process+and+analysis+software+sp0550/global+lab+image+program+sp0550/us07308077-110-20-16
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image process and analysis software sp0550 - by Bioz Stars, 2026-10
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Ruskinn Technology Ltd ruskinn-baker invivo2 400
FIGURE 4. The enhanced inhibitory effect of NECA is mediated by the enhanced contribution of A2AR-mediated signaling and the decreased contribution of A3R-mediated signaling. Unstimulated and activated microglia were exposed to 100 ng LPS/ml in the presence or absence of 10 M NECA in combination with A1, A2A, A2B, and <t>A3</t> receptor antagonists. After 16 h, TNF- (A) and IL-12p40/p70 (B) levels were measured and expressed as relative values of exposure to 100 ng LPS/ml alone. DPCPX was used as an A1R, SCH58261 as an A2AR, alloxazine as an A2BR and VUF5574 as an A3R antagonist. All antagonists were used at 10 M concentrations. Solid lines in the graph indicate relative cytokine production levels of exposure to 100 ng LPS/ml 10 M NECA without antagonists of unstimulated microglia, the dashed lines indicate activated microglia. , p 0.05; Student’s t test. TNF- (C) and IL-12p40/p70 (D) production after exposure to 100 ng LPS/ml in the presence or absence of 10 M CGS21680, an A2AR-selective agonist, and/or of 10 M VUF5574, an A3R antagonist. Cytokine production is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. , p 0.05; Student’s t test. TNF- (E) and IL-12p40/p70 (F) production after exposure to 100 ng LPS/ml different concentrations of CGS21680, in the presence or absence of A3R antagonist, is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. All data are representative for three independent experiments using different donors.
Ruskinn Baker Invivo2 400, supplied by Ruskinn Technology Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+process+and+analysis+software+sp0550/invivo2+400/10__1200_slash_jco___24___01266-232-8-8
Average 90 stars, based on 1 article reviews
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ISO 13485 (medical device quality management system) and ISO 9001 certified. Produced in Class 100,000 (partially Class 10,000) GMP cleanrooms with a fully automatic production process. U.S. FDA registered company (registration No.: 3011966385) and obtained
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FIGURE 4. The enhanced inhibitory effect of NECA is mediated by the enhanced contribution of A2AR-mediated signaling and the decreased contribution of A3R-mediated signaling. Unstimulated and activated microglia were exposed to 100 ng LPS/ml in the presence or absence of 10 M NECA in combination with A1, A2A, A2B, and A3 receptor antagonists. After 16 h, TNF- (A) and IL-12p40/p70 (B) levels were measured and expressed as relative values of exposure to 100 ng LPS/ml alone. DPCPX was used as an A1R, SCH58261 as an A2AR, alloxazine as an A2BR and VUF5574 as an A3R antagonist. All antagonists were used at 10 M concentrations. Solid lines in the graph indicate relative cytokine production levels of exposure to 100 ng LPS/ml 10 M NECA without antagonists of unstimulated microglia, the dashed lines indicate activated microglia. , p 0.05; Student’s t test. TNF- (C) and IL-12p40/p70 (D) production after exposure to 100 ng LPS/ml in the presence or absence of 10 M CGS21680, an A2AR-selective agonist, and/or of 10 M VUF5574, an A3R antagonist. Cytokine production is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. , p 0.05; Student’s t test. TNF- (E) and IL-12p40/p70 (F) production after exposure to 100 ng LPS/ml different concentrations of CGS21680, in the presence or absence of A3R antagonist, is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. All data are representative for three independent experiments using different donors.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia.

doi: 10.4049/jimmunol.0803383

Figure Lengend Snippet: FIGURE 4. The enhanced inhibitory effect of NECA is mediated by the enhanced contribution of A2AR-mediated signaling and the decreased contribution of A3R-mediated signaling. Unstimulated and activated microglia were exposed to 100 ng LPS/ml in the presence or absence of 10 M NECA in combination with A1, A2A, A2B, and A3 receptor antagonists. After 16 h, TNF- (A) and IL-12p40/p70 (B) levels were measured and expressed as relative values of exposure to 100 ng LPS/ml alone. DPCPX was used as an A1R, SCH58261 as an A2AR, alloxazine as an A2BR and VUF5574 as an A3R antagonist. All antagonists were used at 10 M concentrations. Solid lines in the graph indicate relative cytokine production levels of exposure to 100 ng LPS/ml 10 M NECA without antagonists of unstimulated microglia, the dashed lines indicate activated microglia. , p 0.05; Student’s t test. TNF- (C) and IL-12p40/p70 (D) production after exposure to 100 ng LPS/ml in the presence or absence of 10 M CGS21680, an A2AR-selective agonist, and/or of 10 M VUF5574, an A3R antagonist. Cytokine production is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. , p 0.05; Student’s t test. TNF- (E) and IL-12p40/p70 (F) production after exposure to 100 ng LPS/ml different concentrations of CGS21680, in the presence or absence of A3R antagonist, is expressed relative to production after exposure to 100 ng LPS/ml alone for unstimulated and activated microglia. All data are representative for three independent experiments using different donors.

Article Snippet: Cells were washed and incubated for 30 min with anti-adenosine A3 receptor Abs (SP055P, Acris Antibodies) or total rabbit IgG (AbD Serotec) as an isotype control.

Techniques: